QuicKey Pro Rat T(Testosterone)ELISA Kit

Elabscience
Product Code: E-OSEL-R0003
Product Group: ELISA Kits
Supplier: Elabscience
CodeSizePrice
E-OSEL-R0003-24T24T£188.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-OSEL-R0003-48T48T£285.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-OSEL-R0003-96T96T£355.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 6 months. After test the unused wells and reagents should be stored according to the storage table in the manual.

Images

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Standard curve

Standard curve

Documents

Further Information

Abbreviation:
T
Assay Time:
1.5h
Cross reactivity:
This kit recognizes Rat T in samples.No significant cross-reactivity or interference between Rat T and analogues was observed
Detection Range:
0.16-10 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.07 ng/mL
Target Species:
Rat
Target Synonym:
Testosterone;T;
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat T are added to the micro ELISA plate wells. Rat T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The concentration of Rat T in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma
Type:
Competitive
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat T concentrations in serum, plasma.

References

  • https://www.sciencedirect.com/science/article/pii/S0144861722002089
  • https://www.sciencedirect.com/science/article/pii/S004896972100262X
  • https://www.mdpi.com/2076-3921/11/10/1879
  • https://www.sciencedirect.com/science/article/pii/S0147651322010892
  • https://www.hindawi.com/journals/omcl/2022/2113293/
  • https://www.sciencedirect.com/science/article/pii/S026974911935955X
  • https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8439717/
  • https://www.sciencedirect.com/science/article/pii/S0753332222002591
  • https://www.hindawi.com/journals/omcl/2021/7382900/
  • https://europepmc.org/article/pmc/pmc9520912?javascript_support=no
  • https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo01744f
  • https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7993663/