QuicKey Pro Rat ADP/Acrp30(Adiponectin) ELISA Kit

Elabscience
Product Code: E-OSEL-R0006
Product Group: ELISA Kits
Supplier: Elabscience
CodeSizePrice
E-OSEL-R0006-24T24T£188.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-OSEL-R0006-48T48T£285.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-OSEL-R0006-96T96T£355.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 6 months. After test the unused wells and reagents should be stored according to the storage table in the manual.

Images

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Standard curve

Standard curve

Documents

Further Information

Abbreviation:
ADP;Acrp30
Assay Time:
1.5h
Cross reactivity:
This kit recognizes Rat ADP/Acrp30 in samples.No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed
Detection Range:
31.25-1000 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
6.43 ng/mL
Target Species:
Rat
Target Synonym:
Adiponectin;GBP28;ACDC;APM1;ADPN;AdipoQ;ADIPQTL1;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ADP/Acrp30 are added to the micro ELISA plate wells. Rat ADP/Acrp30 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ADP/Acrp30.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The OD value is proportional to the concentration of Rat ADP/Acrp30. The concentration of Rat ADP/Acrp30 in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma
Type:
Sandwich
UNIProt ID:
A0A0G2K845
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat ADP/Acrp30 concentrations in serum, plasma.

References

  • https://www.mdpi.com/1999-4923/14/7/1368
  • https://www.mdpi.com/1871912
  • https://www.mdpi.com/1414304
  • https://www.mdpi.com/989394
  • https://www.mdpi.com/2076-3921/9/11/1073
  • https://www.mdpi.com/1422-0067/22/10/5400
  • https://pubmed.ncbi.nlm.nih.gov/34305591/
  • https://www.frontiersin.org/articles/10.3389/fcimb.2021.740236/full
  • https://www.sciencedirect.com/science/article/pii/S0960076022001303
  • https://www.sciencedirect.com/science/article/pii/S0899900722003264
  • https://www.mdpi.com/2131052
  • https://www.sciencedirect.com/science/article/pii/S0955286320304903