Human D2D(D-Dimer) ELISA Kit

Elabscience
Product Code: E-EL-H6145
Product Group: ELISA Kits
Supplier: Elabscience
CodeSizePrice
E-EL-H6145-24T24T£207.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-EL-H6145-96T96T£317.00£403.00
  • Special Offer! 20% off selected Elabscience ELISA Kits. Read more here.
  • Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-EL-H6145-48T48T£321.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
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Overview

Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month store the items separately according to the storage table in the manual once the kit is received.

Images

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Standard curve

Standard curve

Documents

Further Information

Abbreviation:
D2D
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Human D2D in samples.No significant cross-reactivity or interference between Human D2D and analogues was observed
Detection Range:
6.25-400 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
3.75 ng/mL
Target Species:
Human
Target Synonym:
D dimer
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human D2D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human D2D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Human D2D. You can calculate the concentration of Human D2D in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
plasma
Type:
Sandwich
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human D2D concentrations in plasma.

References

  • https://www.sciencedirect.com/science/article/pii/S1931524417301226
  • https://pubmed.ncbi.nlm.nih.gov/33577011/
  • https://www.sciencedirect.com/science/article/pii/S1567576920314946