Recombinant 2019-nCoV Papain-Like Protease Protein
| Code | Size | Price |
|---|
| PKSR030472-10ug | 10ug | £253.00 |
Quantity:
| PKSR030472-50ug | 50ug | £542.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Virus
Regulatory Status: RUO
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt store it immediately at < -20°C.
Storage:
Store at < -20°C stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
Papain-Like Protease
Accession:
QHD43415.1
Background:
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
Calculated MW:
35.8 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Supplied as a 0.2 uM filtered solution of 20mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
34 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Glu1564-Lys1878
Target Synonym:
Papain-like Protease;PLpro;PL-PRO;pp1a;Replicase polyprotein 1a
UNIProt ID:
P0DTC2
References
- https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.2c02074