Recombinant Human Interleukin-36 alpha/IL-36 alpha
| Code | Size | Price |
|---|
| PKSH033873-20ug | 20ug | £210.00 |
Quantity:
| PKSH033873-100ug | 100ug | £472.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Cell Culture
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
IL-36A;IL-36 alpha
Accession:
Q9UHA7
Activity:
Measured in a cell proliferation assay using A-431 Human epithelial carcinoma cells. The ED50 for this effect is 12.0 ng/ml.
Background:
Human Interleukin-36α (IL-36α) is a secreted cytokine that belongs to the Interleukin 1 cytokine family. IL-36α is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36α is the only IL-1 family member found to be expressed on T-cells. IL-36α and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36α inhibits PPARγ expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this cytokine. IL-36α, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36α may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.
Calculated MW:
17.1 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from a 0.2 um filtered solution of 50mM Tris-HCl, 150mM NaCl, 1 mM EDTA, 0.02% Tween 20, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
17 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Lys6-Phe158
Target Synonym:
Interleukin-36 Alpha;FIL1 Epsilon;Interleukin-1 Epsilon;IL-1 Epsilon;Interleukin-1 Family Member 6;IL-1F6;IL36A;FIL1E;IL1E;IL1F6
UNIProt ID:
Q9UHA7