Recombinant Cynomolgus Signal-Regulatory Protein alpha-1/SIRPA/CD172a (C-6His)
| Code | Size | Price |
|---|
| PKSQ050105-10ug | 10ug | £170.00 |
Quantity:
| PKSQ050105-50ug | 50ug | £355.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Monkey
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
SIRPA;CD172a
Accession:
I7G9Z7
Activity:
Immobilized Cynomolgus SIRPA-His(Cat#PKSQ050105) at 10ug/ml (100 ul/well) can bind Anti-Human SIRPA mAb-Fc. The ED50 of Anti-Human SIRPA mAb-Fc is 17.7 ng/ml.
Background:
Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation
Calculated MW:
37.9 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-6His
ObservedMW:
45-65 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Glu31-Arg369
Target Synonym:
Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1;SHP Substrate 1;SHPS-1;Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs;Bit;CD172 Antigen-Like Family Member A;Inhibitory Feceptor SHPS-1;Macrophage Fusion Receptor;MyD-1 Antigen;Signal-Regulatory Protein Alpha-1;Sirp-Alpha-1;Signal-Regulatory Protein Alpha-2;Sirp-Alpha-2;Signal-Regulatory Protein Alpha-3;Sirp-Alpha-3;p84;CD172a;SIRPA;BIT;MFR;MYD1;PTPNS1;SHPS1;SIRP
UNIProt ID:
I7G9Z7