Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc)
| Code | Size | Price |
|---|
| PKSQ050112-10ug | 10ug | £183.00 |
Quantity:
| PKSQ050112-50ug | 50ug | £392.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Monkey
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
Siglec-15;CD33L3
Accession:
A0A2K5UY47
Activity:
Immobilized Cynomolgus Siglec-15-Fc(Cat#PKSQ050112) at 2ug/ml (100 ul/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 20.9 ng/ml.
Background:
Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
Calculated MW:
53.1 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of 50mM Tris-HCl, 100mM Glycine, 150mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-Fc
ObservedMW:
55-70 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Phe20-Thr263
Target Synonym:
Sialic acid-binding Ig-like lectin 15;Siglec-15;CD33 antigen-like 3;CD33L3
UNIProt ID:
A0A2K5UY47