Recombinant Mouse Siglec-E (C-Fc)
| Code | Size | Price |
|---|
| PKSM041446-10ug | 10ug | £251.00 |
Quantity:
| PKSM041446-50ug | 50ug | £599.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
Siglec-E
Accession:
Q6PJ50
Background:
Siglecs are sialic acid specific I type lectins that are characterized by an extracellular domain (ECD) with an N-terminal Ig-like V-set domain followed by varying numbers of Ig like C2 set domains. Mouse Siglec-E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 - 85 kDa member of the CD33 related subfamily of Siglecs. Rodent and primate Siglec gene families have significantly diverged, and Siglec-9 is the most likely human ortholog of mouse Siglec-E. Siglec-E is expressed as a heavily N-glycosylated disulfide-linked homodimer and shows binding preference for disialic acids in the alpha 2-8 linkage. Siglec-E is up regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists. Siglec-E signaling negatively regulates the LPS-induced production of TNF alpha and IL6 by macrophages. Its up regulation in macrophages parallels the development of endotoxin tolerance. Siglec-E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL12 p40 production.
Calculated MW:
64.4 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-Fc
ObservedMW:
85-95 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Gln20-Phe355
Target Synonym:
SiglecE;Siglec-E;Sialic Acid Binding Ig-like Lectin E
UNIProt ID:
Q6PJ50