Recombinant Human 5'-Nucleotidase/5'-NT/CD73 (AAH65937.1, C-Fc)
| Code | Size | Price |
|---|
| PKSH033885-10ug | 10ug | £255.00 |
Quantity:
| PKSH033885-50ug | 50ug | £612.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt store it immediately at < -20°C.
Storage:
Store at < -20°C stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
5'-Nucleotidase;5'-NT;CD73
Accession:
AAH65937.1
Activity:
Immobilized Human CD73-Fc at 2ug/ml (100 ul/well) can bind Anti-Human CD73 mAb : Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD73 mAb is 0.31 ug/ml.
Background:
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Calculated MW:
84.6 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Supplied as a 0.2 um filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-Fc
ObservedMW:
85-100 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Trp27-Lys547
Target Synonym:
5'-Nucleotidase;5'-NT;Ecto-5'-Nucleotidase;CD73;NT5E;NT5;NTE