Recombinant Human CD3 epsilon/CD3E (C-mFC)
| Code | Size | Price |
|---|
| PKSH033869-10ug | 10ug | £183.00 |
Quantity:
| PKSH033869-50ug | 50ug | £392.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
CD3 epsilon;CD3E
Accession:
P07766
Activity:
Immobilized Human CD3E-mFc at 10ug/ml (100 ul/well) can bind Human Anti-CD3. The ED50 of Human CD3E-mFc is 9.4 ng/ml.
Background:
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Calculated MW:
28.5 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-mFC
ObservedMW:
32-40 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Gln22-Thr48
Target Synonym:
T-Cell Surface Glycoprotein CD3 Epsilon Chain;T-Cell Surface Antigen T3/Leu-4 Epsilon Chain;CD3e;CD3E;T3E
UNIProt ID:
P07766