Recombinant Human Siglec-10 (C-mFc)
| Code | Size | Price |
|---|
| PKSH033911-10ug | 10ug | £182.00 |
Quantity:
| PKSH033911-50ug | 50ug | £392.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
Siglec-10
Accession:
Q96LC7
Activity:
Immobilized Anti-Human Siglec10 mAb at 2ug/ml (100 ul/well) can bind Human Siglec-10-mFc(Cat#PKSH033911). The ED50 of Human Siglec-10-mFc is 18.16 ng/ml.
Background:
Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
Calculated MW:
84.6 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-mFc
ObservedMW:
110-120 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Met17-Thr546
Target Synonym:
SIGLEC10;MGC126774;PRO940;Siglec10;SLG2;sialic acid-binding Ig-like lectin 10;Siglec-10;siglec-like gene 2;Siglec-like protein 2;SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7
UNIProt ID:
Q96LC7