ATF3 Polyclonal Antibody

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Elabscience
Product Code: E-AB-66081
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-66081-60uL60uL£210.00
Quantity:
E-AB-66081-120uL120uL£291.00
Quantity:
E-AB-66081-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of 293T cells using ATF3 Polyclonal Antibody at 1:1000 dilution.
2 / 4
Western blot analysis of extracts of RAW264.7 cells using ATF3 Polyclonal Antibody at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 ug/ml) at 37°C for 8 hours.
3 / 4
Immunohistochemistry of paraffin-embedded human lung cancer using ATF3 Polyclonal antibody at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 4
Immunofluorescence analysis of U-2 OS cells using ATF3 Polyclonal antibody at dilution of 1:100. Blue:DAPI for nuclear staining.
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Western blot analysis of extracts of 293T cells using ATF3 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of RAW264.7 cells using ATF3 Polyclonal Antibody at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 ug/ml) at 37°C for 8 hours.
Immunohistochemistry of paraffin-embedded human lung cancer using ATF3 Polyclonal antibody at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U-2 OS cells using ATF3 Polyclonal antibody at dilution of 1:100. Blue:DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
ATF3
Background:
This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
12kDa/13kDa/14kDa/15kDa/20kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human ATF3
ObservedMW:
23KDa/24KDa
Purification method:
Affinity purification
Target Synonym:
ATF3
UNIProt ID:
P18847