MRPS35 Polyclonal Antibody

Elabscience
Product Code: E-AB-66610
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-66610-60uL60uL£210.00
Quantity:
E-AB-66610-120uL120uL£291.00
Quantity:
E-AB-66610-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using MRPS35 Polyclonal Antibody at dilution of 1:1000.
2 / 2
Immunofluorescence analysis of L929 cells using MRPS35 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines using MRPS35 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using MRPS35 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
MRPS35
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
21kDa/36kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human MRPS35 (NP_068593.2).
ObservedMW:
37kDa
Purification method:
Affinity purification
Target Synonym:
MRPS35;HDCMD11P;MDS023;MRP-S28;MRPS28
UNIProt ID:
P82673