Flag-Tag Monoclonal Antibody

Elabscience
Product Code: E-AB-20028
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-20028-30uL30uL£125.00
Quantity:
E-AB-20028-60uL60uL£146.00
Quantity:
E-AB-20028-120uL120uL£196.00
Quantity:
E-AB-20028-200uL200uL£216.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: Clone:9K2
Regulatory Status: RUO
Application: Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western Blot analysis of Recombinant protein using Flag-Tag Monoclonal Antibody at dilution of 1) 1:5000, 2) 1:10000.

Western Blot analysis of Recombinant protein using Flag-Tag Monoclonal Antibody at dilution of 1) 1:5000, 2) 1:10000.

Documents

Further Information

Abbreviation:
Flag-Tag
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-10000
Immunogen:
Synthetic Peptide
Purification method:
Protein A purification
Target Synonym:
DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag