Flag-Tag Monoclonal Antibody
| Code | Size | Price |
|---|
| E-AB-20006-30uL | 30uL | £125.00 |
Quantity:
| E-AB-20006-60uL | 60uL | £146.00 |
Quantity:
| E-AB-20006-120uL | 120uL | £196.00 |
Quantity:
| E-AB-20006-200uL | 200uL | £216.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: Clone:4A2
Regulatory Status: RUO
Applications:
- Immunofluorescence (IF)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
Flag-Tag
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300
Immunogen:
Synthetic Peptide
Purification method:
Protein A purification
Target Synonym:
DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag
References
- https://www.frontiersin.org/articles/10.3389/fpls.2021.713544/full