GFP Monoclonal Antibody

Elabscience
Product Code: E-AB-20090
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-20090-30uL30uL£125.00
Quantity:
E-AB-20090-60uL60uL£146.00
Quantity:
E-AB-20090-120uL120uL£196.00
Quantity:
E-AB-20090-200uL200uL£216.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: Clone:4A1
Regulatory Status: RUO
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western Blot analysis of 1) GFP Mock (control), 2) GFP+ target protein using GFP Monoclonal Antibody at dilution of 1:5000.
2 / 2
1) Input: Hela Cells, 2) IP product: IP at dilution of  1:200

Western Blot analysis of 1) GFP Mock (control), 2) GFP+ target protein using GFP Monoclonal Antibody at dilution of 1:5000.
1) Input: Hela Cells, 2) IP product: IP at dilution of  1:200

Documents

Further Information

Abbreviation:
GFP
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:3000-10000
Immunogen:
Recombinant Protein
Purification method:
Protein A purification
Target Synonym:
GFP;Green fluorescent protein