Elabscience

GFP Monoclonal Antibody

Product Code:
 
E-AB-20086
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Mouse
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
Clone:3I3
Regulatory Status:
 
RUO
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western Blot analysis of GFP transfected Hela cells using GFP Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.
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Western Blot analysis of GFP transfected Hela cells using GFP Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.

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E-AB-20086-30uL30uL£125.00
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E-AB-20086-60uL60uL£146.00
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E-AB-20086-120uL120uL£196.00
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E-AB-20086-200uL200uL£216.00
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This product comes from: China.
Typical lead time: 14-21 working days.
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  • Further Information
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Further Information

Abbreviation:
GFP
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:5000-1:10000, IP 1:100-1:300
Immunogen:
Recombinant Protein
Purification method:
Protein A purification
Target Synonym:
GFP;Green fluorescent protein

Documents

References

  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840182/
  • https://www.mdpi.com/2076-3921/9/12/1193