SMAD7 Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-53271-20uL | 20uL | £125.00 |
Quantity:
| E-AB-53271-60uL | 60uL | £172.00 |
Quantity:
| E-AB-53271-120uL | 120uL | £237.00 |
Quantity:
| E-AB-53271-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
SMAD7
Background:
SMAD7,also named as Mothers against decapentaplegic homolog 7,is a 426 amino acid protein,which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1,which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Buffer:
PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Calculated MW:
46 kDa
Concentration:
1.14 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:40-1:200, ELISA 1:5000-1:10000
Immunogen:
Synthetic peptide of human SMAD7
ObservedMW:
Refer to figures
Purification method:
Antigen affinity purification
Target Synonym:
CRCS3;FLJ16482;hSMAD 7;hSMAD7;MAD (mothers against decapentaplegic Drosophila) homolog 7;MAD;Mad homolog 7;MAD homolog 8;MAD mothers against decapentaplegic homolog 7;MADH 7;MADH 8;MADH6;MADH8;Mothers Against Decapentaplegic Drosophila Homolog of 6;Mothers Against Decapentaplegic Drosophila Homolog of 7;Mothers against decapentaplegic homolog 7;Mothers against decapentaplegic homolog 8;Mothers against DPP homolog 7;Mothers against DPP homolog 8;SMA- AND MAD-RELATED PROTEIN 7;SMAD 7;SMAD;SMAD family member 7;SMAD;mothers against DPP homolog 7 (Drosophila);SMAD;mothers against DPP homolog 7;SMAD6;Smad7;SMAD7
UNIProt ID:
O15105