GCLC Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-52359-20uL | 20uL | £125.00 |
Quantity:
| E-AB-52359-60uL | 60uL | £172.00 |
Quantity:
| E-AB-52359-120uL | 120uL | £237.00 |
Quantity:
| E-AB-52359-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
GCLC
Background:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Buffer:
PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Calculated MW:
73 kDa
Concentration:
0.8 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000
Immunogen:
Fusion protein of human GCLC
ObservedMW:
Refer to figures
Purification method:
Antigen affinity purification
Target Synonym:
EC 6.3.2.2;Gamma ECS;Gamma glutamylcysteine synthetase;Gamma-ECS;Gamma-glutamylcysteine synthetase;GCL;Gclc;GCS;GCS heavy chain;GLCL;GLCLC;Glutamate cysteine ligase catalytic subunit;Glutamate--cysteine ligase catalytic subunit;GSH1
UNIProt ID:
P48506
References
- https://www.sciencedirect.com/science/article/pii/S2213231721002007
- https://www.mdpi.com/1170076