beta actin Polyclonal Antibody

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Elabscience
Product Code: E-AB-40338
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-40338-30uL30uL£125.00
Quantity:
E-AB-40338-60uL60uL£146.00
Quantity:
E-AB-40338-120uL120uL£196.00
Quantity:
E-AB-40338-200uL200uL£216.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000
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Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200
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Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200
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Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200
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Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000
Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Documents

Further Information

Abbreviation:
ACTBB
Background:
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Buffer:
PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
Calculated MW:
41 kDa
Concentration:
0.85mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:3000-1:30000 IHC 1:100-1:300
Immunogen:
Recombinant Zebrafish Actin, cytoplasmic 2 protein
ObservedMW:
41 kDa
Purification method:
Antigen Affinity Purification
Target Synonym:
ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A
UNIProt ID:
Q7ZVF9

References

  • https://www.sciencedirect.com/science/article/pii/S2162253120301724
  • https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb00114d
  • https://www.mdpi.com/2073-4409/10/10/2647
  • https://www.mdpi.com/2072-6694/13/9/2066
  • https://www.mdpi.com/2073-4409/10/5/1013
  • https://www.mdpi.com/1905846
  • https://www.mdpi.com/1905046
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7293
  • https://www.sciencedirect.com/science/article/pii/S1567576922007597
  • https://cancerci.biomedcentral.com/articles/10.1186/s12935-020-1132-3
  • https://www.sciencedirect.com/science/article/pii/S0944711321003883
  • https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgac065/6726478