GAPDH Polyclonal Antibody

Contact us
Elabscience
Product Code: E-AB-40337
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-40337-30uL30uL£125.00
Quantity:
E-AB-40337-60uL60uL£146.00
Quantity:
E-AB-40337-120uL120uL£196.00
Quantity:
E-AB-40337-200uL200uL£216.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000
2 / 3
Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400
3 / 3
Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400
❮❯

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000
Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Documents

Further Information

Abbreviation:
GAPDH
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Buffer:
PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
Calculated MW:
35 kDa
Concentration:
0.66mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:3000-1:6000 IHC 1:300-1:500
Immunogen:
Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
ObservedMW:
35 kDa
Purification method:
Antigen Affinity Purification
Target Synonym:
aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component
UNIProt ID:
Q5XJ10

References

  • https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btm2.10275
  • https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3919938
  • https://pubmed.ncbi.nlm.nih.gov/35860242/
  • https://www.sciencedirect.com/science/article/pii/S0753332222006783
  • https://www.mdpi.com/1579044
  • https://www.sciencedirect.com/science/article/pii/S0223523422003117
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019468/
  • https://www.sciencedirect.com/science/article/pii/S1567576922004763
  • https://www.sciencedirect.com/science/article/pii/S1567576923000449
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053591/
  • https://iovs.arvojournals.org/article.aspx?articleid=2783840
  • https://www.sciencedirect.com/science/article/pii/S1567576921000928