Recombinant Streptomyces hygroscopicus Bar Protein
| Code | Size | Price |
|---|
| PKSQ050087-10ug | 10ug | £180.00 |
Quantity:
| PKSQ050087-50ug | 50ug | £378.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Bacteria
Regulatory Status: RUO
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
Bar
Accession:
P16426
Background:
Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
Calculated MW:
20.6 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
18-20 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met1-Ile183
Target Synonym:
Phosphinothricin N-acetyltransferase;PPT N-acetyltransferase;Phosphinothricin-resistance protein;bar
UNIProt ID:
P16426