Recombinant Rabbit TNF-alpha/TNFSF2/TNFa Protein
| Code | Size | Price |
|---|
| PKSQ050066-10ug | 10ug | £192.00 |
Quantity:
| PKSQ050066-50ug | 50ug | £411.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Regulatory Status: RUO
Application: Cell Culture
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
TNF-alpha;TNFSF2;TNFa
Accession:
P04924
Activity:
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3. 5 pg/ml.
Background:
Tumor necrosis factor alpha (TNFα) is the prototypic ligand of the TNF superfamily. TNFα forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1,p55R) and TNF-R2 (TNF receptor type 2,p75R). TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Calculated MW:
17.59 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 300mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
18 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Val77-Leu235
Target Synonym:
Tumor Necrosis Factor;Cachectin;TNF-Alpha;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;TNF;TNFA;TNFSF2
UNIProt ID:
P04924