Recombinant Mouse TRAIL R2/TNFRSF10B Protein (Fc Tag)(Active)
| Code | Size | Price |
|---|
| PKSM041271-10ug | 10ug | £170.00 |
Quantity:
| PKSM041271-50ug | 50ug | £315.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Cell Culture
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
TRAIL R2;TNFRSF10B
Accession:
Q9QZM4
Activity:
Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 92.04 ng/ml in the presence of 40 ng/mL of TNFSF10.
Background:
Mouse tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is a member of the TNFR family which contains 1 death domain and 3 TNFR-Cys repeats. TNFRSF10B exhibits high structural and functional homology to TRAIL-R1 (DR-4). TNFRSF10B is highly expressed in heart, lung, lymphocytes, spleen and kidney. In addition, it is regulated by the tumor suppressor p53. TNFRSF10B is the receptor for the cytotoxic ligand TNFSF10/TRAIL. It promotes the activation of NF-kappa-B and is essential for ER stress-induced apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases mediating apoptosis.
Calculated MW:
40.9 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM PB,150mM NaCl,pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-Fc
ObservedMW:
50-75 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Asn53-Ser177
Target Synonym:
Tumor Necrosis Factor Receptor Superfamily Member 10B;Death Receptor 5;TNF-Related Apoptosis-Inducing Ligand Receptor 2;TRAIL Receptor 2;TRAIL-R2;CD262;TNFRSF10B;DR5;KILLER;TRAILR2;TRICK2;ZTNFR9
UNIProt ID:
Q9QZM4