Recombinant Mouse PD-L1/B7-H1/CD274 Protein (His Tag)
| Code | Size | Price |
|---|
| PKSM041248-10ug | 10ug | £126.00 |
Quantity:
| PKSM041248-50ug | 50ug | £180.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
PD-L1;B7-H1;CD274
Accession:
Q9EP73
Activity:
Immobilized Anti-Human PDL1 mAb-Fc at 2ug/ml (100 ul/well)can bind Mouse PD-L1-His. The ED50 of Mouse PD-L1-His is 23.35 ng/ml.
Background:
Mouse Programmed cell death 1 ligand 1(Cd274,PD-L1), is a member of the growing B7 family of immune proteins.It involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors. B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs.B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.
Calculated MW:
25.6 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-6His
ObservedMW:
39-58 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Phe19-Thr238
Target Synonym:
Programmed cell death 1 ligand 1Cd274;programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;programmed death ligand 1;B7 homolog 1;B7-H1;CD274;B7h1;Pdcd1l1;Pdcd1lg1;Pdl1
UNIProt ID:
Q9EP73