Recombinant Human Interleukin-18/IL-18 Protein
| Code | Size | Price |
|---|
| PKSH033626-20ug | 20ug | £210.00 |
Quantity:
| PKSH033626-100ug | 100ug | £472.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Cell Culture
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
Interleukin-18;IL-18
Accession:
Q14116
Activity:
Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is < 5 ng/mL.
Background:
Interleukin-18 is a secreted protein and it belongs to the IL-1 family. IL-18 is a proinflammatory cytokine and produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. After stimulation with IL-18; natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells.
Calculated MW:
18.2 kDa
Endotoxin:
< 0.01 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from 1xPBS, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
17 kDa
Purity:
> 98 % as determined by reducing SDS-PAGE.
Sequence:
Tyr37-Asp193
Target Synonym:
Interleukin-18;Iboctadekin;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;GIF;IL-18;IL-1g;IL1F4;MGC12320;IL18
UNIProt ID:
Q14116