Recombinant Human BDNF Protein (with pro peptide)(Active)
| Code | Size | Price |
|---|
| PKSH033559-10ug | 10ug | £180.00 |
Quantity:
| PKSH033559-50ug | 50ug | £378.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
BDNF
Accession:
P23560
Activity:
Immobilized Human pro-BDNF at 2ug/ml(100 ul/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human pro-BDNF is 1. 4 ug/ml.
Background:
The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis; whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
Calculated MW:
25.6 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Supplied as a 0.2 um filtered solution of 20mM PB, 10% Trehalose, 100mM NaCl, 0.02% Tween 80, pH 6.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
28 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Ala19-Arg247(R125A,R127A,R128A)
Target Synonym:
Brain-Derived Neurotrophic Factor;BDNF;Abrineurin
UNIProt ID:
P23560