Recombinant Human UBE2M/UBC12 Protein
| Code | Size | Price |
|---|
| PKSH033478-10ug | 10ug | £226.00 |
Quantity:
| PKSH033478-50ug | 50ug | £283.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt store it immediately at < -20°C.
Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
UBE2M;UBC12
Accession:
P61081
Background:
UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. This protein is linked with a ubiquitin-like protein; NEDD8; which can be conjugated to cellular proteins; such as Cdc53/culin. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1; but not RBX2; suggests that the RBX1-UBE2M complex neddylates specific target proteins; such as CUL1; CUL2; CUL3 and CUL4. It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Calculated MW:
20.9 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Supplied as a 0.2 um filtered solution of 50mM HEPES, pH 7.5, 2mM DTT, 150mM NaCl, 10% Glycerol.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
25 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met1-Lys183
Target Synonym:
NEDD8-conjugating enzyme Ubc12;NEDD8 carrier protein;NEDD8 protein ligase;Ubiquitin-conjugating enzyme E2 M;UBC12;UBE2M;
UNIProt ID:
P61081