Total Bile Acid (TBA) Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K181-M-48T | 48T | £197.00 |
Quantity:
| E-BC-K181-M-96T | 96T | £237.00 |
Quantity:
| E-BC-K181-M-500Assays | 500Assays | £639.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
25 min
Detection Instrument:
Microplate reader
Detection Range:
2.05-120 umol/L
Detection Type:
Colorimetric method
Inter cv:
3.7
Intra cv:
3.4
Result Type:
Quantitative
Sensitivity:
2.05 umol/L
Target Synonym:
TBA
Test Principle:
With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
Tested Sample Types:
serum, animal tissue
References
- https://www.mdpi.com/2098480
- https://www.sciencedirect.com/science/article/pii/S2212429222005399
- https://www.mdpi.com/1861292
- https://www.hindawi.com/journals/ecam/2022/7832540/