F-TESTO(Free Testosterone) ELISA Kit
| Code | Size | Price |
|---|
| E-EL-0165-24T | 24T | £183.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-EL-0165-48T | 48T | £275.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
| E-EL-0165-96T | 96T | £342.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Further Information
Abbreviation:
F-TESTO
Assay Time:
2.5h
Cross reactivity:
This kit recognizes Universal F-TESTO in samples.No significant cross-reactivity or interference between Universal F-TESTO and analogues was observed
Detection Range:
31.25-2000 pg/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
18.75 pg/mL
Target Species:
Universal
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal F-TESTO. During the reaction, Universal F-TESTO in the sample or standard competes with a fixed amount of Universal F-TESTO on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal F-TESTO. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Universal F-TESTO in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma
Type:
Competitive
Usage:
This ELISA kit applies to the in vitro quantitative determination of F-TESTO concentrations in serum, plasma.
References
- https://www.sciencedirect.com/science/article/pii/S0147651321004371
- https://www.sciencedirect.com/science/article/pii/S1319562X21003909
- https://www.sciencedirect.com/science/article/pii/S0890623815300113
- https://www.sciencedirect.com/science/article/pii/S0887233319305478
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025583/
- https://link.springer.com/article/10.1007/s12011-017-1023-1
- https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.12490
- https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.12439
- https://www.sciencedirect.com/science/article/pii/S1642431X22000328
- http://www.publish.csiro.au/RD/RD18505
- https://onlinelibrary.wiley.com/doi/abs/10.1111/and.13137
- https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12930