TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit

Elabscience
Product Code: E-EL-0162
Product Group: ELISA Kits
Supplier: Elabscience
CodeSizePrice
E-EL-0162-24T24T£183.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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E-EL-0162-48T48T£275.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-EL-0162-96T96T£291.00£342.00
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  • Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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Overview

Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Images

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Standard curve

Standard curve

Documents

Further Information

Abbreviation:
TGF-β1
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Universal TGF-β1 in samples.No significant cross-reactivity or interference between Universal TGF-β1 and analogues was observed
Detection Range:
0.16-10 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.1 ng/mL
Target Species:
Universal
Target Synonym:
Cartilage-inducing factor;CED;Differentiation inhibiting factor;DPD1;LAP;Latency-associated peptide;Prepro transforming growth factor beta 1;TGF beta 1;TGF beta;TGF beta 1 protein;TGF-beta 1 protein;TGF-beta-1;TGF-beta-5;TGF-beta1;TGFB;Tgfb-1;tgfb1;TGFB1;TGFbeta;TGFbeta1;Transforming Growth Factor b1;Transforming Growth Factor beta 1;Transforming growth factor beta 1a;transforming growth factor beta-1;transforming growth factor;beta 1;Transforming Growth Factor-?1;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Universal TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Universal TGF-β1. You can calculate the concentration of Universal TGF-β1 in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
H:P01137;M:P04202;R:P17246
Usage:
This ELISA kit applies to the in vitro quantitative determination of TGF-β1 concentrations in Serum, plasma and other biological fluids.

References

  • https://www.sciencedirect.com/science/article/pii/S1385894722059460
  • https://www.sciencedirect.com/science/article/pii/S0142961222003258
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/all.15163
  • https://www.sciencedirect.com/science/article/pii/S1385894722011925
  • https://link.springer.com/article/10.1007/s42765-022-00224-w
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201661
  • https://pubs.acs.org/doi/abs/10.1021/acsami.2c13976
  • https://pubs.acs.org/doi/abs/10.1021/acsami.2c14448
  • https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01461-2
  • https://www.sciencedirect.com/science/article/pii/S0168365921002996
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/