(KO Validated) Vimentin Polyclonal Antibody

Elabscience
Product Code: E-AB-63601
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-63601-60uL60uL£224.00
Quantity:
E-AB-63601-120uL120uL£317.00
Quantity:
E-AB-63601-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells using Vimentin Polyclonal Antibody at dilution of 1:1000.
2 / 2
Immunofluorescence analysis of L929 cells using Vimentin Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells using Vimentin Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using Vimentin Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
Vimentin
Background:
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
53kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Vimentin (NP_003371.2).
ObservedMW:
57kDa
Purification method:
Affinity purification
Target Synonym:
CTRCT30;HEL113;Vimentin;VIM;vimentin
UNIProt ID:
P08670