RPA2 Polyclonal Antibody

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Elabscience
Product Code: E-AB-60671
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-60671-60uL60uL£210.00
Quantity:
E-AB-60671-120uL120uL£291.00
Quantity:
E-AB-60671-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using RPA2 Polyclonal Antibody at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.
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Immunohistochemistry of paraffin-embedded human kidney cancer using RPA2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Western blot analysis of extracts of various cell lines using RPA2 Polyclonal Antibody at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.
Immunohistochemistry of paraffin-embedded human kidney cancer using RPA2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
RPA2
Background:
As part of the heterotrimeric replication protein A complex (RPA/RP-A, binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
29kDa/30kDa/38kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human RPA2
ObservedMW:
32kDa
Purification method:
Affinity purification
Target Synonym:
REPA2;RP-A p32;RP-A p34;RPA32;RPA2;RP-Ap32;RP-Ap34
UNIProt ID:
P15927