(KO Validated) ROCK1 Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-62198-60uL | 60uL | £224.00 |
Quantity:
| E-AB-62198-120uL | 120uL | £317.00 |
Quantity:
| E-AB-62198-200uL | 200uL | £465.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
ROCK1
Background:
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
158kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:100
Immunogen:
Recombinant fusion protein of human ROCK1 (NP_005397.1).
ObservedMW:
158kDa
Purification method:
Affinity purification
Target Synonym:
ROCK1;P160ROCK;ROCK-I
UNIProt ID:
Q13464