RAF1 Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-60020-60uL | 60uL | £210.00 |
Quantity:
| E-AB-60020-120uL | 120uL | £291.00 |
Quantity:
| E-AB-60020-200uL | 200uL | £431.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
RAF1
Background:
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
73kDa/75kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human RAF1 (NP_002871.1).
ObservedMW:
73kDa
Purification method:
Affinity purification
Target Synonym:
CMD1NN;CRAF;NS5;Raf-1;c-Raf;RAF1
UNIProt ID:
P04049
References
- https://febs.onlinelibrary.wiley.com/doi/abs/10.1111/febs.16359