PCNA Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-70004-60uL | 60uL | £184.00 |
Quantity:
| E-AB-70004-120uL | 120uL | £251.00 |
Quantity:
| E-AB-70004-200uL | 200uL | £351.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
Proliferating Cell Nuclear Antigen
Background:
Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.
Buffer:
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Calculated MW:
33kDa
Concentration:
270 ug/mL
Conjugation:
Unconjugated
Dilution:
WB 1:1000-1:3000, IHC 1:300-1:800
Immunogen:
KLH conjugated Synthetic peptide corresponding to Mouse PCNA
ObservedMW:
33kDa
Purification method:
Affinity purification
Target Synonym:
ATLD2;cb16;Cyclin;DNA polymerase delta auxiliary protein;etID36690.10;fa28e03;fb36g03;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA;PCNAR;Polymerase delta accessory protein;Proliferating cell nuclear antigen;wu:fa28e03;wu:fb36g03
UNIProt ID:
P12004,P17918,P04961