NEDD8 Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-60222-60uL | 60uL | £210.00 |
Quantity:
| E-AB-60222-120uL | 120uL | £291.00 |
Quantity:
| E-AB-60222-200uL | 200uL | £431.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
NEDD8
Background:
Nedd8 is an 81 amino acid Ub-like protein. It is conjugated to protein substrates in much the same way as Ub, using the E1, E2, E3 cascade. The specific E1 used is a heterodimer of APPBP1/UBA3. Two E2s have been discovered to follow called Ubc12 and Ube2F. Neddylation causes conformational change of the Cullin protein in the E3 called cullin-RING ligases (CRLs), which enhances the E3 Ub ligase activity of CRLs.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
9kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000
Immunogen:
Recombinant fusion protein of human NEDD8
ObservedMW:
12kDa
Purification method:
Affinity purification
Target Synonym:
NEDD8;NEDD-8
UNIProt ID:
Q15843