Lamin A/C Polyclonal Antibody

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Elabscience
Product Code: E-AB-60043
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-60043-60uL60uL£210.00
Quantity:
E-AB-60043-120uL120uL£291.00
Quantity:
E-AB-60043-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using Lamin A/C Polyclonal Antibody at dilution of 1:1000. HeLa cells were treated by Hydroxyurea (4 mM) at 37?C for 20 hours or treated by Paclitaxel (100 nM/ml) at 37?C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37?C for 20 hours or treated by Paclitaxel (100 nM) at 37?C for 20 hours.
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Immunohistochemistry of paraffin-embedded Rat brain using Lamin A/C Polyclonal Antibody at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse kidney using Lamin A/C Polyclonal Antibody at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens).
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Confocal immunofluorescence analysis of A-431 cells using Lamin A/C Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
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Confocal immunofluorescence analysis of U-2 OS cells using Lamin A/C Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody
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Western blot analysis of extracts of various cell lines using Lamin A/C Polyclonal Antibody at dilution of 1:1000. HeLa cells were treated by Hydroxyurea (4 mM) at 37?C for 20 hours or treated by Paclitaxel (100 nM/ml) at 37?C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37?C for 20 hours or treated by Paclitaxel (100 nM) at 37?C for 20 hours.
Immunohistochemistry of paraffin-embedded Rat brain using Lamin A/C Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using Lamin A/C Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens).
Confocal immunofluorescence analysis of A-431 cells using Lamin A/C Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2 OS cells using Lamin A/C Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody

Documents

Further Information

Abbreviation:
Lamin A/C
Background:
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
62-74kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Lamin C (NP_005563.1).
ObservedMW:
69kDa/72kDa
Purification method:
Affinity purification
Target Synonym:
LMNA;CDCD1;CDDC;CMD1A;CMT2B1;EMD2;FPL;FPLD;FPLD2;HGPS;IDC;LDP1;LFP;LGMD1B;LMN1;LMNC;LMNL1;MADA;PRO1;lamin;Lamin A/C
UNIProt ID:
P02545