FEN1 Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-60232-60uL | 60uL | £210.00 |
Quantity:
| E-AB-60232-120uL | 120uL | £291.00 |
Quantity:
| E-AB-60232-200uL | 200uL | £431.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
FEN1
Background:
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
35kDa/42kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:10-1:100
Immunogen:
Recombinant fusion protein of human FEN1 (NP_004102.1).
ObservedMW:
48kDa
Purification method:
Affinity purification
Target Synonym:
FEN1;FEN-1;MF1;RAD2
UNIProt ID:
P39748