EIF3A Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-62282-60uL | 60uL | £210.00 |
Quantity:
| E-AB-62282-120uL | 120uL | £291.00 |
Quantity:
| E-AB-62282-200uL | 200uL | £431.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
EIF3A
Background:
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
162kDa/166kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:1000,IF 1:50-1:100
Immunogen:
Recombinant fusion protein of human EIF3A
ObservedMW:
180kDa
Purification method:
Affinity purification
Target Synonym:
EIF3A;EIF3;EIF3S10;P167;TIF32;eIF3-p170;eIF3-theta;p180;p185
UNIProt ID:
Q14152