CAD Polyclonal Antibody

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Elabscience
Product Code: E-AB-62648
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-62648-60uL60uL£210.00
Quantity:
E-AB-62648-120uL120uL£291.00
Quantity:
E-AB-62648-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using CAD Polyclonal Antibody at dilution of 1:1000.
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Immunohistochemistry of paraffin-embedded Human liver using CAD Polyclonal Antibody at dilution of 1:100 (40x lens).
3 / 8
4 / 8
Immunohistochemistry of paraffin-embedded Human tonsil using CAD Polyclonal Antibody at dilution of 1:100 (40x lens).
5 / 8
Immunohistochemistry of paraffin-embedded Rat testis using CAD Polyclonal Antibody at dilution of 1:100 (40x lens).
6 / 8
Immunohistochemistry of paraffin-embedded Mouse spleen using CAD Polyclonal Antibody at dilution of 1:100 (40x lens).
7 / 8
Immunofluorescence analysis of NIH-3T3 cells using CAD Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using CAD Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using CAD Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human liver using CAD Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human tonsil using CAD Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat testis using CAD Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spleen using CAD Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using CAD Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CAD Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
CAD
Background:
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
242kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human CAD (NP_004332.2).
ObservedMW:
243kDa
Purification method:
Affinity purification
Target Synonym:
CAD;CDG1Z;EIEE50;GATD4
UNIProt ID:
P27708