Brdu Monoclonal Antibody

Elabscience
Product Code: E-AB-70248
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-70248-60uL60uL£184.00
Quantity:
E-AB-70248-120uL120uL£251.00
Quantity:
E-AB-70248-200uL200uL£351.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 4B6C12
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemistry analysis of paraffin-embedded mouse jejunum (intraperitoneal injection BrdU every 2 hours for 4 times) using Brdu Monoclonal Antibody at dilution of 1:300.
2 / 2
Immunofluorescence analysis of Hela cells (treated with 0.03mg/ml BrdU for 40 min) using Brdu Monoclonal Antibody at dilution of 1:100.

Immunohistochemistry analysis of paraffin-embedded mouse jejunum (intraperitoneal injection BrdU every 2 hours for 4 times) using Brdu Monoclonal Antibody at dilution of 1:300.
Immunofluorescence analysis of Hela cells (treated with 0.03mg/ml BrdU for 40 min) using Brdu Monoclonal Antibody at dilution of 1:100.

Documents

Further Information

Abbreviation:
Brdu
Background:
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. 

Buffer:
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Concentration:
0.7 mg/mL
Conjugation:
Unconjugated
Dilution:
IHC 1:100-1:500, IF 1:100-1:500
Immunogen:
KLH conjugated BrdU
Purification method:
Affinity purification
Target Synonym:
Bromodeoxyuridine;Bromodeoxyuridine;5-Bromo-2-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.