ATG5 Polyclonal Antibody

Elabscience
Product Code: E-AB-60008
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-60008-60uL60uL£210.00
Quantity:
E-AB-60008-120uL120uL£291.00
Quantity:
E-AB-60008-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using ATG5 Polyclonal Antibody at dilution of 1:1000.
2 / 2
Immunofluorescence analysis of HeLa cells using ATG5 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines using ATG5 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of HeLa cells using ATG5 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
ATG5
Background:
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
22kDa/32kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human ATG5 (NP_004840.1).
ObservedMW:
55kDa
Purification method:
Affinity purification
Target Synonym:
ATG5;APG5;APG5-LIKE;APG5L;ASP;hAPG5
UNIProt ID:
Q9H1Y0