AMPKα2 Polyclonal Antibody

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Elabscience
Product Code: E-AB-63857
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-63857-60uL60uL£224.00
Quantity:
E-AB-63857-120uL120uL£317.00
Quantity:
E-AB-63857-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using AMPKα2 Polyclonal Antibody at dilution of 1:1000.
2 / 4
Immunofluorescence analysis of C6 cells using AMPKα2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of HeLa cells using AMPKα2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of NIH-3T3 cells using AMPKα2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using AMPKα2 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of C6 cells using AMPKα2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using AMPKα2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using AMPKα2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
AMPKα2
Background:
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
62kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human AMPKα2 (NP_006243.2).
ObservedMW:
69kDa
Purification method:
Affinity purification
Target Synonym:
PRKAA2;AMPK;AMPK2;AMPKa2;PRKAA
UNIProt ID:
P54646

References

  • https://www.sciencedirect.com/science/article/pii/S2162253119303981