ACAT1 Polyclonal Antibody

Contact us
Elabscience
Product Code: E-AB-64523
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-64523-60uL60uL£210.00
Quantity:
E-AB-64523-120uL120uL£291.00
Quantity:
E-AB-64523-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 7
Western blot analysis of extracts of various cell lines using ACAT1 Polyclonal Antibody at dilution of 1:5000.
2 / 7
Immunohistochemistry of paraffin-embedded Human liver damage using ACAT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded Human liver cancer using ACAT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded Rat ovary using ACAT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
5 / 7
Immunohistochemistry of paraffin-embedded Mouse kidney using ACAT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
6 / 7
Immunofluorescence analysis of NIH-3T3 cells using ACAT1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of U-2 OS cells using ACAT1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines using ACAT1 Polyclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded Human liver damage using ACAT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human liver cancer using ACAT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat ovary using ACAT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using ACAT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using ACAT1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ACAT1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
ACAT1
Background:
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
17kDa/45kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human ACAT1 (NP_000010.1).
ObservedMW:
42kDa
Purification method:
Affinity purification
Target Synonym:
ACAT1;ACAT;MAT;T2;THIL
UNIProt ID:
P24752