MEK(Methionine-Enkephalin) ELISA Kit
| Code | Size | Price |
|---|
| E-EL-0020-24T | 24T | £183.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-EL-0020-48T | 48T | £275.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
| E-EL-0020-96T | 96T | £342.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Further Information
Abbreviation:
MEK
Assay Time:
2.5h
Cross reactivity:
This kit recognizes Universal MEK in samples.No significant cross-reactivity or interference between Universal MEK and analogues was observed
Detection Range:
125-8000 pg/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
75 pg/mL
Target Species:
Universal
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal MEK. During the reaction, Universal MEK in the sample or standard competes with a fixed amount of Universal MEK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal MEK. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Universal MEK in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Competitive
UNIProt ID:
P01210
Usage:
This ELISA kit applies to the in vitro quantitative determination of MEK concentrations in serum, plasma and other biological fluids.
References
- https://www.spandidos-publications.com/10.3892/mmr.2020.11501
- https://link.springer.com/article/10.1186/s12871-019-0912-6