FA/VB9(Folic Acid/Vitamin B9) ELISA Kit
| Code | Size | Price |
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| E-EL-0009-24T | 24T | £183.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-EL-0009-48T | 48T | £275.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
| E-EL-0009-96T | 96T | £291.00£342.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Further Information
Abbreviation:
FA;VB9
Assay Time:
2.5h
Cross reactivity:
This kit recognizes Universal FA/VB9 in samples.No significant cross-reactivity or interference between Universal FA/VB9 and analogues was observed
Detection Range:
1.56-100 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.94 ng/mL
Target Species:
Universal
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal FA/VB9. During the reaction, Universal FA/VB9 in the sample or standard competes with a fixed amount of Universal FA/VB9 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal FA/VB9. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Universal FA/VB9 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Competitive
Usage:
This ELISA kit applies to the in vitro quantitative determination of FA/VB9 concentrations in serum, plasma and other biological fluids.
References
- https://academic.oup.com/ecco-jcc/article-abstract/9/9/708/346040
- https://pubs.rsc.org/--/content/articlehtml/2016/nr/c6nr00946h
- https://www.hindawi.com/journals/omcl/2022/9171684/
- https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8529044/
- https://www.nature.com/articles/s41598-017-01732-1
- https://www.karger.com/Article/Abstract/517911
- https://europepmc.org/article/med/32498709?javascript_support=no
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689552/
- https://www.tandfonline.com/doi/abs/10.1080/08039488.2019.1582697
- https://www.sciencedirect.com/science/article/pii/S0044848614004025
- https://onlinelibrary.wiley.com/doi/abs/10.1111/anu.12275
- https://www.ajol.info/index.php/ahs/article/view/195031