Recombinant Mouse TNF-alpha/TNFA Protein (aa 80-235)(Active)
| Code | Size | Price |
|---|
| PKSM041240-20ug | 20ug | £210.00 |
Quantity:
| PKSM041240-100ug | 100ug | £472.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Cell Culture
Shipping:
The product is shipped at ambient temperature. Upon receipt store it immediately at the storage temperature listed.
Storage:
Store at < -20°C stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
TNF-alpha;TNFA
Accession:
NP_038721.1
Activity:
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Background:
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. TNF-alpha Protein & Antibody
Calculated MW:
17 kDa
Expression Host:
E.coli
Formulation:
Lyophilized from sterile 50mM Tris 0.2M NaCl, pH 7.2
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
17 kDa
Purity:
> 98 % as determined by reducing SDS-PAGE.
Sequence:
Leu 80-Leu 235
Target Synonym:
Tumor Necrosis Factor;Cachectin;TNF-Alpha;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;Tumor Necrosis Factor;Membrane Form;Tumor Necrosis Factor;Tnf;Tnfsf2;DIF;TNF-alpha;Tnfa;TNFalpha;Tnfsf1a;TNFSF2