Recombinant Mouse SCF/c-Kit Ligand Protein (Active)
| Code | Size | Price |
|---|
| PKSM041151-10ug | 10ug | £219.00 |
Quantity:
| PKSM041151-50ug | 50ug | £340.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Cell Culture
Shipping:
The product is shipped at ambient temperature. Upon receipt store it immediately at the storage temperature listed.
Storage:
Store at < -20°C stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
SCF;c-Kit Ligand
Accession:
P20826
Activity:
Measured by the dose-dependent stimulation of TF-1 cells. The ED50 for this effect is 4-12 ng/mll.
Background:
Mouse stem cell factor (SCF), is the ligand for the receptor-type protein-tyrosine kinase KIT. It plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. It also promotes phosphorylation of PIK3R1, which is the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5.
Calculated MW:
18.4 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
16 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Lys26-Ala189
Target Synonym:
FPH2;KIT ligand;Kitl;KITLG;KL-1;Mast cell growth factor;MGF;MGFSHEP7;SCF;Stem cell factor;SFc-Kit ligand;SLF;steel factor;Hematopoietic growth factor KL
UNIProt ID:
P20826