Recombinant Mouse CD47 Protein (His Tag) (Active)
| Code | Size | Price |
|---|
| PKSM041042-10ug | 10ug | £170.00 |
Quantity:
| PKSM041042-50ug | 50ug | £315.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Store at < -20°C stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
CD47
Accession:
Q61735-2
Activity:
Immobilized Mouse SIRPA -Fc(Cat: PKSM041145) at 2ug/ml(100 ul/well) can bind Mouse CD47-His. The ED50 of Mouse CD47-His is 5. 736 ug/mL.
Background:
CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a glycosylated atypical member of the immunoglobulin superfamily. Mouse CD47 is an integral membrane protein that consists of a extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and C-terminal cytoplasmic tail. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It plays an important role in memory formation and synaptic plasticity in the hippocampus. As a receptor for SIRPA, it binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cellcell adhesion, it enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. It may play a role in membrane transport and/or integrin dependent signal transduction. It also prevents premature elimination of red blood cells.
Calculated MW:
16.7 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-6His
ObservedMW:
30-60 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Gln19-Pro158
Target Synonym:
LAP;OA3;Leukocyte Surface Antigen CD47;Antigenic Surface Determinant Protein OA3;Integrin-Associated Protein;Protein MER6;CD47;MER6
UNIProt ID:
Q61735